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BioRad, Hercules, CA, USA) and real-time quantitative PCR (qPCR) was performed using a TaqMan 7900 Sequence Detection System with TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA)

Based on the available data, tirzepatide has the lowest discontinuation rates and similar or slightly lower GI side effect rates compared to semaglutide

There is some evidence in the literature suggesting that suboptimal Se intake during pregnancy is a common phenomenon [24] and that inadequate micronutrient intake during this period may result in poor fetal growth and development as well as poor pregnancy outcome [25]

Refrigeration at 28 C is appropriate once the working timeline extends past a month

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